J Integr Plant Biol. ›› 1964, Vol. 12 ›› Issue (3): -.

• Research Articles •    

Studies on the Growth of Excised Stem Tips of Stachys sieboldii II.

C. L. Lee, Sun An-tze and Y. L. Kwei   

Abstract: The present investigation deals with a comparative study on the growth of excised stem tips of Stachys sieboldii Miq. in vitro. The stem tips were excised from the tubers shortly after germination. Following ordinary cleansing and sterilization, the portions above the fifth node of the stem tips were cultured in basic media containing different concentrations of adenine, bee royal jelly, 2,4-D, indoleacetic acid, naphthylacetic acid, and gibberellin. Observations on the growth pattern following 1, 2, and 4 weeks of culture showed the effect of growth stimulation by the above mentioned organic substances at certain concentrations. Among those, indoleacetic acid and gibberellin of 0.5 ppm., bee royal jelly of 100 ppm and adenine of 1 and 10 ppm showed the most significant influence. Variations in the development of primordia of the adventitious roots were noticed at the base of the excised stem tips treated in vitro. At low concentrations the initiation, organization and extention of the adventitious roots were more or less normal, and the stem tips showed more rapid growth. However, at high concentrations, with the exceptions of those cultured in adenine and bee royal jelly media, the growth of the stem tips and their bases exhibited various degrees of modification, being most prominent in the naphthylacetic acid media. The loci of root initiation were not confined within the “pericycle”. At later stages, the primordia of the adventitious roots showed tumorous aggregations with loss of internal layering. The upper part of the stem tips ceased to extend at high concentrations. The internal layering and zonation disappeared. The apical meristem became parenchymatous. No new leaf primordium was initiated and, instead, those already present were greatly enlarged. Frequently, tumors of various sizes were seen in the young leaves.

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