J Integr Plant Biol. ›› 1963, Vol. 11 ›› Issue (3): -.

• Research Articles •    

Experimental Studies of Young Ginkgo Embryos I. The Effect of the Bee Royal Jelly on the Embryo Growth

F. H. Wang, T. K. Chen and S. C. Lee   

Abstract: Isolated young Ginkgo embryos were grown in vitro under aseptic condition in a basic agar medium containing sucrose, mineral salts, 4 vitamins and glycine. In one series of experiments 3% aqueous extract of Ginkgo female gametophyte and 0.1ppm IAA were also added. The bee royal jelly at different concentrations were tested for its effect on the differentiation and development of the young embryos. The young embryos above 1.5 mm in length with differentiated cotyledons grew normally or produced callus-like growth on the basic medium as well as on the media with the royal jelly added. Part of the young embryos above lmm but without differentiation of cotyledons may continue their normal differentiation and development on the media containing the royal jelly while not one embryo of the same size range can grow normally on the basic medium. Young embryos below 1 mm usually showed little growth and died after three weeks of culture. The most effective concentration of bee royal jelly is about 400 ppm. It appears that some substances which affect the normal differentiation and growth of young Ginkgo embryos are present in the bee royal jelly.

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