J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (6): 681-687.

• Research Articles • Previous Articles     Next Articles

Gene Flow from Transgenic Chinese Cabbage (Brassica campestris ssp. chinensis var. utilis) to Related Cruciferae Species Through Outcrossing

LIU Fan, ZHAO Hong, YAO Lei, ZHANG Jun-Jie, CAO Ming-Qing   


It was substantiated by morphological observation, chromosome and DNA identification that the bargene, conferring the resistance to herbicide basta in transgenic Chinese cabbage (Brassica campestris ssp. chinensis var. utilisTsen et Lee), could intrude into the genomes of turnip (B. campestris ssp.rapifera), heading Chinese cabbage (B. campestris ssp. pekinensis) and other non_heading Chinese cabbage (B. campestris ssp. chinensis) in a relatively high frequency while into genome of B. napusat a low frequency via natural cross_pollination in open field. Herbicide resistant hybrids of B. nigra, B. carinata, andB. juncea could be obtained at certain frequency in the greenhouse by hand pollination. At no time did the resistant hybrid plants of transgenic Chinese cabbage with B. oleracea, R. sativus and seven wild weeds, widespread in China, gained in our experiments.

刘凡  赵泓  姚磊  张军杰  曹鸣庆
(1. 北京蔬菜研究中心,北京100089; 北京农业生物技术中心,北京100089)

摘要: 从形态、染色体及分子水平上证实,转基因不结球白菜(Brassica campestris ssp.chinensis var.utilis Tsen et  Lee)中编码除草剂Basta抗性的bar基因能在田间条件下,经自然传粉,以较高频率侵入芜菁(B.campestris ssp.rapifera)、结球白菜(B.campestris ssp.peknensis)和不结球白菜(B.campestrs ssp.chnensis)的基因组中,也能少量侵入同属异种的甘蓝型油菜(B.napus)基因组中;在温室人工辅助授粉条件下,除在上述种中的基因漂移率提高外,bar基因尚能以一定频率侵入同属的黑芥(B.nigra)、埃塞俄比亚芥(B.carnata)、芥菜(B.juncea)基因组中,但始终未能得到转基因白菜与结球甘蓝(B.oleracea)、萝卜(R.sativus)的杂种。转基因白菜与十字花科的7种常见杂草经温室人工辅助授粉,也均未得到抗性杂种。


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