J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (6): 631-637.

• Research Articles •

### Real-Time PCR Technique and Its Application in Quantification of Plant Nucleic Acid Molecules

LIU Jin-Yuan

• Published:2003-06-15

Abstract:

Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of initial template copy numbers as PCR products are generated. This technique significantly simplifies and accelerates the process of producing reproducible quantification of nucleic acid molecules. It not only is a sensitive, accurate and rapid quantitative method, but it also provides an easier way to calculate the absolute starting copy number of nucleic acid molecules to be tested. Together with molecular bio-techniques like microarray, real-time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics. This review introduces the detailed principles and application of the real-time PCR technique, describes a recently developed system for exact quantification of AUX/IAA genes in Arabidopsis, and discusses the problems with the real-time PCR process.

（清华大学生物科学与技术系，北京100084）

Tel: 010-62772243; E-mail: Liujy @ mail.tsinghua.edu.cn。

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