Abstract: In this experiment, we have tried to make a comparison of analytical peroxidase isozyme of waxy maize from South China, using vertical polyacrylamide gel electrophoretic method. 70 cultivars (lines) of the waxy maize and other materials were examined. The main results were summarized as follows. 1. Eighteen of the nineteen waxy maize cultivars from the original region of "Menghai four-row Wax" showed the fifth band of peroxidase isozyme, and one showed mixed band having fourth and fifth band. 2 Among 15 tested waxy maize cultivars from Yunnan Sheng, eleven of them showed the fifth band of peroxidase isozyme, two showed the fourth band and two showed the mixed band. 3. Seventeen of the nineteen waxy maize cultivars from Guangxi Zhuangzu Zizhi- qu showed the fifth band and two showed the mixed band of peroxidase isozyme. Accordingly, conclusion might be drawn that the most (86.8%) of waxy maize cultivars from South China showed the fifth band of peroxidase isozymc. That is similar to Coix which is originated from China. On the contrary, the dent maize from America showed the fourth band. The experimental results further demonstrated that Chinese waxy maize (Zea mays ) might be originated in South China. China is not only one of maize secondary origin centre, but also might be one of primery origin centre of maize.