J Integr Plant Biol. ›› 1981, Vol. 23 ›› Issue (2): -.

• Research Articles •    

Callus Formation and Organ Regeneration in Tissue Culture of Woody Plants

Wang Kai-ji, Zhang Pi-fang, Ni De-xiang and Bao Zi-hua   

Abstract: The tumor-like callus, obtained usually from the surface of explant of woody plant, may be originated from various parts, involving especially epidermis or hypodermis, distal from the cut surface of the explant. These experimental evidences may be used to support the conception of that exogenous hormones play an important role in the induction of callus. The developmental stages of establishment of the callus may be characterized by changes in cell morphology and metabolic condition of the tissue. During the induction or activation phase, a regressive change appears in the peripheral layers of the explant, involving a progress return to a meristematic state denoted by increasing nucleus and nucleolus size and accmnulation of RNA and results in the dedifferentiation of the cells. This is followed by a division stage with substantial accumulation of RNA and active cell division, thus, the peripheral tissue of the explant has been activated and become embryonic tissue and then, the division stage cannot be considered as the phase of regressive change or dedifferentiation. The formation of meristematic nodules in a common feature in developing callus culture and they are originated at random from solitary parenchymatons cells, which by means of dediffercntiation have become meristematic in character. The meristematic

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