Abstract: The fine structure of resin secreting cells of the cortical resin ducts in stem of Pinus sylvestris Carr. was studied with the help of high pressure freezing and freeze-substitution techniques. An unprecedented mode of resin elimination was observed. It seems that the hydrolase-contained Golgi vesicles take part in the dissolution of plasmalemma, and a passage is formed, through which resin material may quickly pass outside of the protoplast. Then, the ruptured plasmalemma was repaired by some vesicles, possibly derived from Golgi bodies.

Key words: Pinus sylvestris, Resin duct and resin elimination, High pressure freezing and freeze-substitution