J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (3): -.

• Research Articles •    

Cloning of the LFY cDNA from Arabidopsis thaliana and Its Transformation to Chrysanthemum morifolium

SHAO Han-Shuang, LI Ji-Hong, ZHENG Xue-Qin and CHEN Shou-Cai   

Abstract: The total length cDNA of Leafy (LFY) was cloned and sequenced from wild type Arabidopsis thaliana (L.) Heynh. The plant expression vector with CaMV 35S promoter for LFY cDNA was constructed and transformed into Chrysanthemum morifolium (Ramat.) Tzvel. The result proved that the LFY eDNA contained 1 263 base pairs in total length, encoding 420 amino acid. After Southern and Northern blot analyses the plants were grown in flowerpot, eight transgenic Ch. morifolium, plants were obtained. Compared with the normal plants, three of them flowered 65,67 and 70 days earlier and two of them delayed flowering 78 and 90 days, respectively.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q