J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (11): -.

• Research Articles •    

Change of Proteins at Different Mitotic Phases in Allium sativum

YU Jian-Chun, PENG Yong-Kang, ZHAO Jian and CHEN Rui-Yang   

Abstract: 100 root tip cells at interphase, prophase, metaphase, anaphase and telophase of mitosis of AUium sativum L. have been isolated respectively and the proteins have been analyzed by combining micromanipulation with micro-SDS-PAGE and ultrasensitive silver staining. Comparing the protein change during the different phases it was shown that different patterns of cyclic changes occurred in various proteins. Some proteins changed with regularity which could be repeated.

Key words: Micro-isolation, Ultrasensitive silver staining, Micro-SDS-PAGE

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