J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (2): -.

• Research Articles •    

Cytological Studies of the Cytoplasmic Inheritance in Lilium regale and L. davidii

LIU Xing-Liang, HU Shi-Yi, ZHANG Jin-Zhong and LONG Ya-Yi   

Abstract: The distribution and characteristics of plastids and mitochondria in the generative and sperm cells of Lilium regale Wils. and L. davidii Duch. were described. In L. regale there were few plastids and abundant mitochondria in the newly formed generative cell. When the generative cell became free in the vegetative cytoplasm, the plastids degenerated completely within the generative cell. It was further proved by DAPI fluorescent technique that there was no organell DNA in the generative cell within the mature pollen grain or the pollen tube. However, distribution of the plastids was strictly polarizable during the division of the micmspore in L. davidii, resulting the lack of plastids in the newly formed generative cell. Data of RFLP analysis comparable between L. davidii, L. longifiorum and their interspecific hybrid have also proved the plastid inheritance in L. davidii to be of uniparental maternal transmission. Although the mitoehondria were observed both in the generative and sperm cells of L. regale and L. davidii but their DNA was decomposed in the male gametophyte stage. Therefore the mitochondda in the sperm cell could not be transmitted into the offspring. The results provided the detail, cytological evidence that organelles in the microgametophyte are incapable of genetic transmission in the two species of Lilium.

Key words: Plastid inheritance, Mitochondrion inheritance, Ultrastmcture, Organell DNA, Lilium regale, Lilium davidii

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