J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (3): -.

• Research Articles •    

Proceeding in Plant Anti-Insect Genetic Engineering

Zhu Xin-sheng and Zhu Yu-xian   

Abstract: The recent proceeding in plant insect resistance genetic engineering was reviewed here. The Bacillus thuringiensis insecticidal crystal protein (Bt-toxins) were the most intensively studied insect toxins. Recent studies mainly concentrated on the isolation of novel Bt-toxins, modification of Bt-toxin by means of code replacement, splicing and enhancing the expressing amount; Proteinase inhibitor genes and lectin genes were promising genes applied in agriculture. The proteinase inhibitor genes such as cowpea trypsin inhibitor kill the pests by inhibiting their digestive function while lectins induced illness of the pests. Some invertebrate such as scorpion and spider can produce some insect toxin targeted on insect selectively. They were another promising insect toxin genes that could be used in agriculture because they were harmless to animal and human being. The modified baculovims with some insect toxins can kill the pests more fast and widely, they were also used in anti-insect engineering. Some problems have arisen in plant anti-insect genetic engineering. Pests evolved the resistance to the transgenic plants in recent years. An effective pathway to solve this problem is to induce more insect toxin genes to one plant at the same time. Another problem is how to enhance the expressing amount of toxin proteins. Those will become the major directions of plant anti-insect genetic engineering.

Key words: Anti-insect plant genetic engineering, Bacillus thuringiensis insecticidal crystal protein, Scorpion insect toxin, Spider insect toxin, Proteinase inhibitor, Lectin, Recombinant baeulovims

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