J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (7): -.

• Research Articles •    

Isolation of Blue-Green Algal Spheroplasts by Penicillin-Lysozyme Method

Guo Hou-liang   

Abstract: Osmotic stabilizer used in present study consists of eight compounds, i.e. ammonium sulfate, ammonium tertrate, KH2PO4, sodium citrate, NH4NO3, NaNO3, KC1 and NaCl. Each of them was dissolved in to 0.5 mol then eight solutions were mixed in equal volume. A menbrane stabilizer CaC12(0.1%) was added to the mixed solution. The pH of the mixed solution was about 6.2. Blue-green algal trichomes for the spheroplast isolation were first treated with penicillin. Penicillin dosage and treating time were different according to different materials. After penicillin procedure, the materials were then treated with lysozyme (0.2%). Temperature of the treatment was 28–30℃, Spheroplast production started in 15 minutes after treatment and finished in 3 hours. The spheroplasts remained a chain form mostly and became single spheroi. d through centrifugation and resuspension. The spheroplasts were typical spherical, more transparent and sensitive to hypotonic condition or mechanical treatment.

Key words: Blue-green algae, Spheroplasts, Penicillin, Lysozyme

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