Induction of G-bands in Root Tip Chromosomes of the Maize

Abstract

Abstract: Authors tried to induce G-bands of chromosomes of root-tips in maize (Zey mays L.. everty Sturt) with a variety of technological modifications. The following techniques were found to be more satisfactory: primary root-tips were treated with an aqueous actinomycin D(AMD) solution (70 μg/ml) at room temperature; air dry slides of chromosomes in maize made the chromosomes spread well and plasma off; and then the preparations of the chromosomes were treated with modified methods of Seabright[7] and Utakoji[8] and the technique of aceto-orcin stain. The G-bands of chromosomes of corn were induced with the three methods above. They were shown in Plate 1, 2, 3 and 4 which are similar to the G-bands of chromosomes in human and mammal, and these bands are more consistent in each chromosomes.

Key words: Maize, Chromosomes G-band

Zhan Tie-sheng, Shi Li-ming and T. C. Hsu. Induction of G-bands in Root Tip Chromosomes of the Maize[J]. J Integr Plant Biol., 1987, 29(5): -.

[1]	Jiong Liu, Lu Li, Zhilong Xiong, Christelle A.M. Robert, Baozhu Li, Shan He, Wenjie Chen, Jiasheng Bi, Guanqing Zhai, Siyi Guo, Hui Zhang, Jieping Li, Shutang Zhou, Xi Zhang and Chun‐Peng Song. Trade-offs between the accumulation of cuticular wax and jasmonic acid-mediated herbivory resistance in maize [J]. J Integr Plant Biol., 2024, 66(1): 143-159.
[2]	Ting Zhang, Shuang‐Hui Zhao, Yan Wang and Yan He. FIGL1 coordinates with dosage-sensitive BRCA2 in modulating meiotic recombination in maize [J]. J Integr Plant Biol., 2023, 65(9): 2107-2121.
[3]	Jie Gao, Long Zhang, Haonan Du, Yongbin Dong, Sihan Zhen, Chen Wang, Qilei Wang, Jingyu Yang, Paifeng Zhang, Xu Zheng and Yuling Li. An ARF24-ZmArf2 module influences kernel size in different maize haplotypes [J]. J Integr Plant Biol., 2023, 65(7): 1767-1781.
[4]	Zhaogui Zhang, Kai Li, Huairen Zhang, Qiuxia Wang, Li Zhao, Juan Liu and Huabang Chen. A single silk‐ and multiple pollen‐expressed PMEs at the Ga1 locus modulate maize unilateral cross‐incompatibility [J]. J Integr Plant Biol., 2023, 65(5): 1344-1355.
[5]	Canrong Ma, Ruoyue Li, Yan Sun, Mou Zhang, Sen Li, Yuxing Xu, Juan Song, Jing Li, Jinfeng Qi, Lei Wang and Jianqiang Wu. ZmMYC2s play important roles in maize responses to simulated herbivory and jasmonate [J]. J Integr Plant Biol., 2023, 65(4): 1041-1058.
[6]	Dexin Qiao, Junya Wang, Min‐Hui Lu, Cuiping Xin, Yiping Chai, Yuanyuan Jiang, Wei Sun, Zhenghong Cao, Siyi Guo, Xue‐Chen Wang and Qi‐Jun Chen. Optimized prime editing efficiently generates heritable mutations in maize [J]. J Integr Plant Biol., 2023, 65(4): 900-906.
[7]	Ming Zhang, Yanhong Chen, Hongyan Xing, Wensheng Ke, Yunlu Shi, Zhipeng Sui, Ruibin Xu, Lulu Gao, Ganggang Guo, Jiansheng Li, Jiewen Xing and Yirong Zhang. Positional cloning and characterization reveal the role of a miRNA precursor gene ZmLRT in the regulation of lateral root number and drought tolerance in maize [J]. J Integr Plant Biol., 2023, 65(3): 772-790.
[8]	Jingyang Gao, Peijiang Feng, Jingli Zhang, Chaopei Dong, Zhao Wang, Mingxiang Chen, Zhongliang Yu, Bowen Zhao, Xin Hou, Huijuan Wang, Zhaokun Wu, Razia Sultana Jemim, Haidong Yu, Doudou Sun, Pei Jing, Jiafa Chen, Weibin Song, Xuecai Zhang, Zijian Zhou and Jianyu Wu, . Enhancing maize's nitrogen-fixing potential through ZmSBT3, a gene suppressing mucilage secretion [J]. J Integr Plant Biol., 2023, 65(12): 2645-2659.
[9]	Yan-Zhuo Yang, Shuo Ding, Xin-Yuan Liu, Chunhui Xu, Feng Sun and Bao-Cai Tan. The DEAD-box RNA helicase ZmRH48 is required for the splicing of multiple mitochondrial introns, mitochondrial complex biosynthesis, and seed development in maize [J]. J Integr Plant Biol., 2023, 65(11): 2456-2468.
[10]	Dongdong Xu, Hailong Yang, Cheng Zou, Wen-Xue Li, Yunbi Xu and Chuanxiao Xie, . Identification and functional characterization of the AGO1 ortholog in maize [J]. J Integr Plant Biol., 2016, 58(8): 749-758.
[11]	Yingni Xiao, Shawn Thatcher, Min Wang, Tingting Wang, Mary Beatty, Gina Zastrow-Hayes, Lin Li, Jiansheng Li, Bailin Li, and Xiaohong Yang. Transcriptome analysis of near-isogenic lines provides molecular insights into starch biosynthesis in maize kernel [J]. J Integr Plant Biol., 2016, 58(8): 713-723.
[12]	Xiaoliang Sun, Weiwei Qi, Yihong Yue, Huiling Ling, Gang Wang, and Rentao Song. Maize ZmVPP5 is a truncated Vacuole H⁺-PPase that confers hypersensitivity to salt stress [J]. J Integr Plant Biol., 2016, 58(6): 518-528.
[13]	Weibin Song, Baobao Wang, Andrew L Hauck, Xiaomei Dong, Jieping Li and Jinsheng Lai. Genetic dissection of maize seedling root system architecture traits using an ultra-high density bin-map and a recombinant inbred line population [J]. J Integr Plant Biol., 2016, 58(3): 266-279.
[14]	Cheng Huang, Qiuyue Chen, Guanghui Xu, Dingyi Xu, Jinge Tian and Feng Tian. Identification and fine mapping of quantitative trait loci for the number of vascular bundle in maize stem [J]. J Integr Plant Biol., 2016, 58(1): 81-90.
[15]	Shangguo Feng, Runqing Yue, Sun Tao, Yanjun Yang, Lei Zhang, Mingfeng Xu, Huizhong Wang, and Chenjia Shen. Genome-wide identification, expression analysis of auxin-responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses [J]. J Integr Plant Biol., 2015, 57(9): 783-795.

Induction of G-bands in Root Tip Chromosomes of the Maize

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments