J Integr Plant Biol. ›› 1987, Vol. 29 ›› Issue (4): -.

• Research Articles •    

Established Haploid and Diploid Embryonic Somaclones of Allooctoploid Triticale

Zheng Qi-cheng, Zhu Yao-lan and Chen Wen-hua   

Abstract: Growing tip of allooctoploid Triticale ‘h739’ plants and its pollen plants at the glume differentiation stage were cultured on MS medium supplemented with 2 mg/l 2,4-D. The callus began to be formed after 20 days. The induction frequency was up to 98.3%. By transferring subcultured calli to regeneration medium plantlet regeneration frequency was 95.2%. Five so- maclones HC-2, HC-3, HC-4, HC-5 and HC-0 have been selected; their plantlet regeneration frequency remained high through long-tern subculture, being more than 90% by 892 days. Most cells in HC-2 and HC-3 were haploidy (2n=28). Histological and scanning electron micros copy studies showed that callus of HC-3 was composed of embryoids at different developmental stages.

Key words: Triticale, Growing tip, Embryoid, Somaclone

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