J Integr Plant Biol. ›› 1986, Vol. 28 ›› Issue (6): -.

• Research Articles •    

Callus Induction and Plant Regeneration from Embryo of Elaeis guineenis Jacq

Cui Yuan-fang, Gong Zheng, Chen Xin-hua, Shao Xing-ti and Zhu Zhi-qing Chu Chih-ching   

Abstract: This paper deals with the preliminary results on callus induction and plant regeration from embryo in vitro of Elaeis guineenis. When mature embryos were cultured on dedifferen- tiation medium they proliferated calli during 30-90 days of culture. Among auxins applied, 2,4-D was more important for callus induction however 2,4-D and NAA combination gave bet- ter result. On the contrary, kinetin inhibited callus formation and growth. These experimental results explain that a higher callus induction frequency depends not only on the constituents of the medium used but also on the genotype of donor plants. After transfering the calli onto kinetin-containing media for a peirad, embryoids, which showed typical configuration of zygotic embryo, could be obtained. The embryoids can further develop into whole plants on a shoot induction medium. Some embryoids have subjected to srveral generations of subculture and still retained the ability to embryoid multification and plant regeneration.

Key words: Callus inductionfrom embryo, Plant regeneration, Elaeis guineenis Jacq.

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