J Integr Plant Biol. ›› 2007, Vol. 49 ›› Issue (8): 1091-1099.DOI: 10.1111/j.1672-9072.2007.00540.x
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Thomas J. Haas and Marisa S. Otegui
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Abstract: This review focuses on the contribution of electron tomography-based techniques to our understanding of cellular processes in plant cells. Electron microscopy techniques have evolved to provide better three-dimensional resolution and improved preservation of the subcellular components. In particular, the combination of cryofixation/freeze substitution and electron tomography have allowed plant cell biologists to image organelles and macromolecular complexes in their native cellular context with unprecedented three-dimensional resolution (4–7 nm). Until now, electron tomography has been applied in plant cell biology for the study of cytokinesis, Golgi structure and trafficking, formation of plant endosome/prevacuolar compartments, and organization of photosynthetic membranes. We discuss in this review the new insights that these tomographic studies have brought to the plant biology field.
Key words: cell structure, electron tomography, high-pressure freezing/freeze substitution.
Thomas J. Haas and Marisa S. Otegui. Electron Tomography in Plant Cell Biology[J]. J Integr Plant Biol., 2007, 49(8): 1091-1099.
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URL: https://www.jipb.net/EN/10.1111/j.1672-9072.2007.00540.x
https://www.jipb.net/EN/Y2007/V49/I8/1091