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J Integr Plant Biol, 1994, 36 (Suppl.): -, Research Article
Relationship Between Polyamines and Morphogenesis in Cotyledons of Cucumis melon L. Cultured in Vitro
Tian Chang-en, Li Ren-gui and Guan He
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Abstract
Cotyledons of Cucumis melon L. would be induced to form callus, adventitous roots and shoots on MS medum with different kinds of and ratios of exogeneous phytohormones. Content of polyamines was measured by means of TLC-fluorometry during different stages of morphogenesis in cultured cotyledons. Effects of D-arg and MGBG [methylgluoxadis (guganylhydrazone) ], which are the inhibitors of polyamine biosynthesis, on endogeneous polyamine and morphogenesis in cultured cotyledons were studied, respectively. The results showed that relationship between the polyamines are not related to callus formaton, but put (putrescine) and all 3 kinds of polyamine measured are related to adventitious root formation. These results indicate that different kinds and ratios of exogeneous phytohormones would change metabolism of the endogeneous polyamine, thus influence the morphogenesis in explant.
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