J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (Suppl.): -.

• Research Articles •    

Relationship Between Polyamines and Morphogenesis in Cotyledons of Cucumis melon L. Cultured in Vitro

Tian Chang-en, Li Ren-gui and Guan He   

  • Published:1994-12-31

Abstract: Cotyledons of Cucumis melon L. would be induced to form callus, adventitous roots and shoots on MS medum with different kinds of and ratios of exogeneous phytohormones. Content of polyamines was measured by means of TLC-fluorometry during different stages of morphogenesis in cultured cotyledons. Effects of D-arg and MGBG [methylgluoxadis (guganylhydrazone) ], which are the inhibitors of polyamine biosynthesis, on endogeneous polyamine and morphogenesis in cultured cotyledons were studied, respectively. The results showed that relationship between the polyamines are not related to callus formaton, but put (putrescine) and all 3 kinds of polyamine measured are related to adventitious root formation. These results indicate that different kinds and ratios of exogeneous phytohormones would change metabolism of the endogeneous polyamine, thus influence the morphogenesis in explant.

Key words: Polyamines, Cucumis melon, Cotyledon, Morphogenesis: In vitro

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22