J Integr Plant Biol. ›› 2018, Vol. 60 ›› Issue (5): 376-381.DOI: 10.1111/jipb.12622

Special Issue: CRISPR

• Letters to the Editor • Previous Articles     Next Articles

Multigene editing via CRISPR/Cas9 guided by a single‐sgRNA seed in Arabidopsis

Zhiming Yu1†, Qiyuan Chen1†, Weiwei Chen1, Xian Zhang1, Fengling Mei1, Pengcheng Zhang1, Mei Zhao1, Xiaohong Wang1, Nongnong Shi1, Stephen Jackson2 and Yiguo Hong1,2,3***   

  1. 1Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
    2Warwick-Hangzhou Joint RNA Signaling Laboratory, School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK
    3Worcester-Hangzhou Joint Molecular Plant Health Laboratory, Institute of Science and the Environment, University of Worcester, WR2 6AJ, UK


We report that a solo single‐guide RNA (sgRNA) seed is capable of guiding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR −associated 9 (CRISRP/Cas9) to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off‐target gene‐editing. Such a gene editing system might be also applicable to modify other homologous genes, or even less‐homologous sequences for multiple gene‐editing in plants and other organisms.

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