J Integr Plant Biol. ›› 2019, Vol. 61 ›› Issue (11): 1121-1127.DOI: 10.1111/jipb.12761

• Letters to the Editor • Previous Articles     Next Articles

The F-box protein HAWAIIAN SKIRT is required for mimicry target-induced microRNA degradation in Arabidopsis

Jun Mei1, Ning Jiang1,2* and Guodong Ren1*   

  1. 1State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China
    2Laboratory of Population & Quantitative Genetics, Institute of Genetics and Biostatistics, School of Life Sciences, Fudan University, Shanghai 200438, China

    Email: Guodong Ren (gdren@fudan.edu.cn, Dr. Ren is fully responsible for the distribution of all materials associated with this article); Ning Jiang (ningjiang@fudan.edu.cn)
  • Received:2018-10-21 Accepted:2018-12-10 Online:2018-12-19 Published:2019-11-01


Mimicry target‐directed microRNA degradation is widespread and highly conserved among eukaryotes. However, little is known about its mechanism of action. In this letter, by using STTM160 (target mimic of miR160) as a reporter, we show that dysfunction of HAWAIIAN SKIRT (HWS) suppresses the pleiotropic phenotype of STTM160. Small RNA sequencing and Northern blot analyses suggested that HWS only affects a subset of microRNAs. Intriguingly, we identified a stable coexistence of miR160/miR399 and their mimicry targets within the AGO1 complex when HWS is compromised, pointing to a possible role of HWS in the clearance of RNA‐induced silencing complexes associated with mimicry target.

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