J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (9): 2204-2217.DOI: 10.1111/jipb.13505

• Plant Biotic Interactions • Previous Articles     Next Articles

Fusarium-produced vitamin B6 promotes the evasion of soybean resistance by Phytophthora sojae

Shuchen Wang1,2,3, Xiaoyi Zhang1,2,3, Zhichao Zhang1,2,3, Yun Chen4, Qing Tian1,2,3, Dandan Zeng1,2,3, Miao Xu1,2,3, Yan Wang1,2,3, Suomeng Dong1,2,3, Zhonghua Ma4, Yuanchao Wang1,2,3, Xiaobo Zheng1,2,3 and Wenwu Ye1,2,3*   

  1. 1. Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China;
    2. Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing 210095, China;
    3. Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, China;
    4. Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
    *Correspondence: Wenwu Ye (yeww@njau.edu.cn)
  • Received:2022-10-10 Accepted:2023-05-10 Online:2023-05-12 Published:2023-09-01

Abstract: Plants can be infected by multiple pathogens concurrently in natural systems. However, pathogen–pathogen interactions have rarely been studied. In addition to the oomycete Phytophthora sojae, fungi such as Fusarium spp. also cause soybean root rot. In a 3-year field investigation, we discovered that P. sojae and Fusarium spp. frequently coexisted in diseased soybean roots. Out of 336 P. sojae–soybean–Fusarium combinations, more than 80% aggravated disease. Different Fusarium species all enhanced P. sojae infection when co-inoculated on soybean. Treatment with Fusarium secreted non-proteinaceous metabolites had an effect equal to the direct pathogen co-inoculation. By screening a Fusarium graminearum mutant library, we identified Fusarium promoting factor of Phytophthora sojae infection 1 (Fpp1), encoding a zinc alcohol dehydrogenase. Fpp1 is functionally conserved in Fusarium and contributes to metabolite-mediated infection promotion, in which vitamin B6 (VB6) produced by Fusarium is key. Transcriptional and functional analyses revealed that Fpp1 regulates two VB6 metabolism genes, and VB6 suppresses expression of soybean disease resistance-related genes. These results reveal that co-infection with Fusarium promotes loss of P. sojae resistance in soybean, information that will inform the sustainable use of disease-resistant crop varieties and provide new strategies to control soybean root rot.

Key words: fungal metabolites, pathogen–host interaction, Phytophthora sojae, soybean root rot, vitamin B6

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