Please wait a minute...

J Integr Plant Biol, 49 (11): 1548-1554, Research Article
Verticase: a Fibrinolytic Enzyme Produced by Verticillium sp. Tj33, an Endophyte of Trachelospermum jasminoides
Ying Li, Jing-Lei Shuang, Wei-Wei Yuan, Wu-Yang Huang and Ren-Xiang Tan
Abstract
Plant endophytes are among the most important resources of biologically active metabolites. Twenty-three endophyte strains residing in Trachelospermum jasminoides were cultivated in vitro with the cultures assayed for the fibrinolytic substance production. As a result, the culture of Verticillium sp. Tj33 was shown to be the most active. A fibrinolytic enzyme designated as verticase was subsequently purified from the supernatant of Verticillium sp. culture broth by a combination of DEAE-52, Sephadex G-75 and hydrophobic column chromatographies. Verticase, with its molecular mass of 31 kDa and pI of 8.5, was demonstrated to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing electrophoresis. Verticase is an enzyme that hydrolyzes fibrin directly without activation of plaminogen. It was stable in a broad pH range from 4 through to 11 with the optimal reaction pH value and temperature shown to be around 9–10 and 50–60 °C, respectively. The fibrinolytic activity of verticase was severely inhibited by phenylmethylsulfony fluoride, indicating that verticase was a serine protease.
---------------------------------------------------
Published: 01 November 2007
© 2007 Institute of Botany, Chinese Academy of Sciences
This Article
   Full Text
   Full Text (PDF)
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Ying Li
   Articles by Jing-Lei Shuang
   Articles by Wei-Wei Yuan
   Articles by Wu-Yang Huang
   Articles by Ren-Xiang Tan
PubMed
   Articles by Ying Li
   Articles by Jing-Lei Shuang
   Articles by Wei-Wei Yuan
   Articles by Wu-Yang Huang
   Articles by Ren-Xiang Tan
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22