Abstract: The promoter region of the rbc S gene from Triticum aestivum L. FAN-61 was amplified by PCR. Two commonly used restriction enzyme sites, Barn HI and Sma Ⅰ, were respectively added to the 5′ and 3′ ends of the promoter fragment in order to make it easier for subsequent manipulation. The amplified fragment was cloned into pUC18/19 vectors and its sequence was determined. The 704 bp promoter fragment contained a TATA Box (TATATATA) and an Ⅰ Box (GATAAT). The sequence of CCAAC in it was similar to the sequence of CCAAT found upstream of other eukaryotic genes. Moreover, it also contained the consensus sequences of CCACA and GAACGTGAGCCA which were present in those of other monocot rbc S.

Key words: Wheat, rbc S promoter, Polymerase chain reaction, Sequencing of nu-Cleotide