J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (8): 688-697.DOI: 10.1111/jipb.12324

• Molecular Physiology • Previous Articles     Next Articles

Development, identification and utilization of introgression lines using Chinese endemic and synthetic wheat as donors

Liqing Gu, Bo Wei, Renchun Fan, Xu Jia, Xianping Wang and Xiangqi Zhang*   

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing, China
  • Received:2014-09-30 Accepted:2014-12-22 Published:2014-12-26
  • About author:These authors contributed equally to this work.
    *Correspondence: E-mail: xqzhang@genetics.ac.cn

Abstract:

Chromosome segmental introgression lines (ILs) are an effective way to utilize germplasm resources in crops. To improve agronomic traits of wheat cultivar (Triticum aestivum) Shi 4185, four sets of ILs were developed. The donors were Chinese endemic subspecies accessions Yunnan wheat (T. aestivum ssp. yunnanense) YN3, Tibetan semi-wild wheat (T. aestivum ssp. tibetanum) XZ-ZM19450, and Xinjiang wheat (T. aestivum ssp. petropavlovskyi) XJ5, and synthetic wheat HC-XM1620 derived from a cross between T. durum acc. D67.2/P66.270 with Aegilops tauschii acc. 218. Totals of 356, 366, 445 and 457 simple sequence repeat (SSR) markers were polymorphic between Shi 4185 and YN3, XZ-ZM19450, XJ5 and HC-XM1620, respectively. In total, 991 ILs were identified, including 300 derived from YN3, covering 95% of the genome of Shi 4185, 218 from XZ-ZM19450 (79%), 279 from XJ5 (97%), and 194 from HC-ZX1620 (84%). The sizes and locations of each introgression were determined from a consensus SSR linkage map. Using the ILs, 11 putative quantitative trait loci (QTLs) were identified for plant height (PH), spike length (SL) and grain number per spike (GNS). Comparative analyses of 24 elite ILs with the parents revealed that the four donor parents could be important resources to improve wheat SL and GNS. Our work offers a case for utilizing endemic landraces for QTL mapping and improvement of wheat cultivars using introgression lines.

 

Gu L, Wei B, Fan R, Jia X, Wang X, Zhang X (2015) Development, identification and utilization of introgression lines using Chinese endemic and synthetic wheat as donors. J Integr Plant Biol 57: 688–697 doi: 10.1111/jipb.12324

Key words: Chromosome segmental introgression lines, common wheat, molecular breeding, quantitative trait locus, simple sequence repeat marker

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