J Integr Plant Biol. ›› 2005, Vol. 47 ›› Issue (6): -.DOI: 10.1111/j.1744-7909.2005.00051.x

• Research Articles •    

Molecular Cytogenetic Identification of a Wheat-Thinopyron intermedium (Host) Barkworth & DR Dewey Partial Amphiploid Resistant to Powdery Mildew

Shu-Bing LIU, Hong-Gang WANG, Xue-Yong ZHANG, Xing-Feng LI, Da-Yong LI, Xia-Yu DUAN and Yi-Lin ZHOU   

Abstract: Wide cross and molecular cytogenetic methods were used to transfer the powdery mildew resistance gene from Thinopyron intermedium (Host) Barkworth & DR Dewey to wheat. Among the progeny of crossing common wheat (Triticum aestivum L.) Yannong 15 with Th. intermedium, a partial amphiploid E990256, with resistance to powdery mildew, was developed. It had 56 chromosomes and could form 28 bivalents in pollen mother cells at metaphase I of meiosis. Resistance verification by race 15 at the seedling stage and by mixed strains of Erysiphales gramnis DC. f. sp. tritici Em. Marchal at the adult stage showed it was immune to powdery mildew at both stages. Gene postulation via 21 isolates of E. gramnis f. sp. tritici and 29 differential hosts showed it was nearly immune to all the isolates used, and its resistance pattern was different from all the mildew resistance genes used, which indicated it probably contained a new resistance gene to powdery mildew. Biochemical verification showed it might convey different Th. intermedium chromosomes from those of the wheat-Th. intermedium partial amphiploids Zhong 1-5. Genomic in situ hybridization analysis by using St genomic DNA as the probe showed E990256 contained a recombination genome of St and E.

Key words: Genomic in situ hybridization (GISH), partial amphiploid, powdery mildew, Thinopyron intermedium, wheat.

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