J Integr Plant Biol. ›› 2006, Vol. 48 ›› Issue (5): 584-590.DOI: 10.1111/j.1744-7909.2006.00285.x
• Research Articles • Next Articles
Yan-Xia Guo, Yan-Sheng Zhang and Yong-Biao Xue
Abstract: Self-incompatibility (SI) is a genetic mechanism of self/non-self pollen recognition to prevent self-fertilization in many flowering plants and, in most cases, this is controlled by a multi-allelic S-locus. S-RNase and S-locus F box (SLF) proteins have been shown to be the female and male determinants of gametophytic self-incompatibility (GSI), respectively, in the Solanaceae, Scrophulariaceae and Rosaceae. Nevertheless, it is thought that additional factors are required for the SI response. Herein, we constructed a mature anther cDNA library from a self-incompatible Petunia hybrida Vilm. line of the S3S3 haplotype. Using AhS2-RNase from Antirrhinum hispanicum as a bait for yeast two-hybrid screening, we found that petunia germinating pollen (PGP) S/D3 was capable of interacting physically with the bait. However, the interaction lacked haplotype specificity. The PGPS/D3 gene is a single copy gene that is expressed in tissues such as the style, ovary, pollen, and leaf. The PGPS/D3::GFP (green fluorescence protein) construct was detected in both the membrane and cytoplasm. The implications of these findings in the operation of S-RNase-based SI are discussed.(Author for correspondence.Tel: 0(10) 6255 2880;Fax: 0(10) 6253 7814; E-mail:ybxue@genetics.ac.cn)
Key words: petunia germinating pollen (PGP) S/D3, Petunia hybrida, self-incompatibility, S-RNase.
Yan-Xia Guo, Yan-Sheng Zhang and Yong-Biao Xue. Petunia Germinating Pollen S/D3 Interacts with S-RNases in Petunia hybrida Vilm.[J]. J Integr Plant Biol., 2006, 48(5): 584-590.
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URL: https://www.jipb.net/EN/10.1111/j.1744-7909.2006.00285.x
https://www.jipb.net/EN/Y2006/V48/I5/584