J Integr Plant Biol. ›› 2006, Vol. 48 ›› Issue (9): -.DOI: 10.1111/j.1744-7909.2006.00299.x

• Research Articles •    

Development of Diagnostic Microscopic and Chemical Markers of Some Euphorbia Latexes

Uppuluri Venkata Mallavadhani, Kantamreddi Venkata Siva Satyanarayana, Anita Mahapatra, Akella Venkata Subrahmanya Sudhakar, Kilambi Narasimhan, Devendar Kumar Pandey and Manikkannan Thirunavokkarasu   

Abstract: The latexes of the three Euphorbia species, namely E. antiquorum L., E. nerifolia L., and E. tirucalli L., are highly valued in the Indian system of medicine as purgatives, in addition to their specific and distinct therapeutic activities. In order to distinguish these latexes and develop their diagnostic microscopic and chemical markers, we performed extensive chemical and microscopic studies. The three latexes differ significantly in their microscopic features by exhibiting characteristic starch grain patterns. Although amoebic structures were found to be characteristic of E. antiquorum, dumb-bell and oval structures are characteristic of E. nerifolia and E. tirucalli, respectively. In addition, these latexes showed bone-shaped structures as a common feature, but these differed considerably in their length (10–60, 30–55, and 50–70 μm in length in E. antiquorum, E. nerifolia, and E. tirucalli, respectively). The chemical markers nerifoliene and euphol were found to be common to both E. antiquorum and E. nerifolia, whereas euphol is the only marker for E. tirucalli. A reverse-phase high-performance thin-layer chromatographic (HPTLC) method was developed to distinguish these three latexes and to generate their standard fingerprinting patterns. Most significantly, the markers nerifoliene and euphol could be resolved by RP-18 F254s precoated aluminium plates and the latexes have been quantitatively estimated with respect to these markers. The developed microscopic, chemical and HPTLC patterns can be used to distinguish the three latexes..(*Author for correspondence. Tel: +91 674 2581 635 ext. 319/249; Fax: +91 674 2581 637; E-mail: uvmavadani@yahoo.com)

Key words: euphol, Euphorbia antiquorum, Euphorbia nerifolia, Euphorbia tirucalli, high-performance thin-layer chromatography, latex, nerifoliene.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q