Please wait a minute...
J Integr Plant Biol, 2006, 48 (12): -, Research Article
Cloning, E. coli Expression and Molecular Analysis of Amorpha-4,11-Diene Synthase from a High-Yield Strain of Artemisia annua L.
Zhen-Qiu Li, Yan Liu, Ben-Ye Liu, Hong Wang, He-Chun Ye and Guo-Feng Li
doi: 10.1111/j.1744-7909.2006.00381.x
Abstract
Increasing demand of artemisinin in the treatment of malaria has placed substantial stress on the total artemisinin supplies world-wide, so more attention has been paid to increasing the content of artemisinin in the Artemisia annua L. plant. In this study, amorpha-4, 11-diene synthase (ADS) cDNA (ads1) and genomics gene (gads1) were cloned from a high-yield A. annua strain 001. The activity of ADS1 was confirmed by heterogeneous overexpression of ads1 and in vitro enzymatic incubation. Reverse transcript-polymerase chain reaction results demonstrated that ads1 expressed in leaves, flowers and young stems, but not in roots. This organ-specific expression pattern of ads1 is consistent with that of artemisinin accumulation in the plant. The gads1 has a complex organization including seven exons and six introns, and belongs to class III terpene synthase. DNA gel blotting revealed that the ADS gene has at least four copies in the genome of strain 001. The higher copy numbers might be one of the reasons for its high artemisinin content.(Author for correspondence. Tel: +86 (0)10 6283 6249; Fax: +86 (0)10 8259 1016; E-mail: hcye@ibcas.ac.cn)
--------------------------------------------------------------------------------------------------------------------
   Online on:
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Zhen-Qiu Li
   Articles by Yan Liu
   Articles by Ben-Ye Liu
   Articles by Hong Wang
   Articles by He-Chun Ye
   Articles by Guo-Feng Li
PubMed
   Articles by Zhen-Qiu Li
   Articles by Yan Liu
   Articles by Ben-Ye Liu
   Articles by Hong Wang
   Articles by He-Chun Ye
   Articles by Guo-Feng Li
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22