J Integr Plant Biol. ›› 2007, Vol. 49 ›› Issue (5): -.DOI: 10.1111/j.1744-7909.2007.00470.x

• Development & Photosynthesis •    

Alterations of RNA Editing for the Mitochondrial ATP9 Gene in a New orf220-type Cytoplasmic Male-sterile Line of Stem Mustard (Brassica juncea var. tumida)

Jing-Hua Yang, Ming-Fang Zhang and Jing-Quan Yu   

Abstract: RNA editing for the mitochondrial ATP9 gene of encoding regions has been observed in both cytoplasmic male-sterile and maintainer lines of stem mustard, where its editing capacity varied spatially and temporally in the cytoplasmic male sterility (CMS) line. There were four RNA editing sites for the mitochondrial ATP9 gene according to its normal editing sites in mustard, of which three sites occurred as C-to-U changes and one as a U-to-C change. As a result, the hydrophobicity of deduced ATP9 protein was reduced due to the conversions at its 17th, 45th and 64th positions. Meanwhile, the conservation of deduced ATP9 protein was enhanced by changes at the 56th position. Loss of a specific editing site for ATP9 was observed in juvenile roots, senile roots, senile leaves and floret buds of the cms line. Comparatively, complete RNA editing for ATP9 gene was retained in juvenile roots, juvenile leaves and floret buds of its maintainer line; however, the loss of a specific editing site for ATP9 gene occurred at senile roots and senile leaves in its maintainer line. These observations allow us to produce a hypothesis that the dysfunction of a specific mitochondrial gene arising from RNA editing could probably be a factor triggering CMS and organ senescence through unknown cross-talk pathways during development.

Key words: ATP9, Brassica juncea, cytoplasmic male sterility, RNA editing.

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