J Integr Plant Biol. ›› 2007, Vol. 49 ›› Issue (12): 1670-1680.DOI: 10.1111/j.1744-7909.2007.00582.x

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Use of Random Amplified Polymorphic DNA Analysis for Economically Important Food Crops

Halima Hassan Salem, Bahy Ahmed Ali, Tian-Hua Huang, Da-Nian Qin, Xiao-Mei Wang and Qing-Dong Xie   

Abstract: The objective of this review is to summarize numerous studies on the use of the random amplified polymorphic DNA (RAPD) technique on rice, corn, wheat, sorghum, barley, rye, and oats to examine its feasibility and validity for assessment of genetic variation, population genetics, mapping, linkage and marker assisted selection, phylogenetic analysis, and the detection of somaclonal variation. Also we discuss the advantages and limitations of RAPD. Molecular markers have entered the scene of genetic improvement in different fields of agricultural research. The simplicity of the RAPD technique made it ideal for genetic mapping, plant and animal breeding programs, and DNA fingerprinting, with particular utility in the field of population genetics.

Key words: random amplified polymorphic DNA, food crops, poaceae.

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