J Integr Plant Biol. ›› 2008, Vol. 50 ›› Issue (4): 452-456.DOI: 10.1111/j.1744-7909.2008.00644.x

• Omics & Epigenetics • Previous Articles     Next Articles

Discrimination of Repetitive Sequences Polymorphism in Secale cereale by Genomic In Situ Hybridization-Banding

Jian-Ping Zhou, Zu-Jun Yang, Guang-Rong Li, Cheng Liu and Zheng-Long Ren   

  • Received:2006-12-03 Accepted:2007-02-13

Abstract: Genomic in situ hybridization banding (GISH-banding), a technique slightly modified from conventional GISH, was used to probe the Chinese native rye (Secale cereale L.) DNA, and enabled us to visualize the individual rye chromosomes and create a universal reference karyotype of the S. cereale chromosome 1R to 7R. The GISH-banding approach used in the present study was able to discriminate S. cereale chromosomes or segments in the wheat (Triticum aestivum L.) background, including the Triticale, wheat-rye addition and translocation lines. Moreover, the GISH-banding pattern of S. cereale subsp. Afghanicum chromosomes was consistent with that of Chinese native rye cv. Jingzhou rye; whereas the GISH-banding pattern of Secale vavilovii was different from that of S. cereale, indicating that GISH-banding can be used to study evolutionary polymorphism in species or subspecies of Secale. In addition, the production and application of GISH-banding to the study of adenine-thymine-riched heterochromatin is discussed.

Key words: Jian-Ping Zhou, Zu-Jun Yang, Guang-Rong Li, Cheng Liu and Zheng-Long Ren

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