J Integr Plant Biol. ›› 2008, Vol. 50 ›› Issue (6): 752-760.DOI: 10.1111/j.1744-7909.2008.00671.x

Special Issue: Cell Biology and Functions

• Development & Photosynthesis • Previous Articles     Next Articles

Assembly of the Protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into New Protoplasts

Demao Li, Fang Lü, Guangce Wang and Baicheng Zhou   

Abstract: The cell organelles of the coenocytic alga Codium fragile (Sur.) Hariot aggregated rapidly and protoplasts were formed when its protoplasm was extruded out in seawater. Continuous observation showed that there were long and gelatinous threads connecting the cell organelles. The threads contracted, and thus the cell organelles aggregated into protoplasmic masses. The enzyme digestion experiments and Coomassie Brilliant Blue and Anthrone stainings showed that the long and gelatinous threads involved in the formation of the protoplasts might include protein and saccharides as structure components. Nile Red staining indicated that the protoplast primary envelope was non-lipid at first, and then lipid materials integrated into its surface gradually. The fluorescent brightener staining indicated that the cell wall did not regenerate in the newly formed protoplasts and they all disintegrated within 72 h after formation. Transmission electron microscopy of the cell wall of wild C. fragile showed electron-dense material embedded in the whole cell wall at regular intervals. The experiments indicated that C. fragile would be a suitable model alga for studying the formation of protoplasts.

Key words: Codium fragile, protoplasm, protoplast, cell wall.

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