J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (4): 374-381.DOI: 10.1111/j.1744-7909.2008.00777.x

• Cell and Developmental Biology • Previous Articles     Next Articles

Interaction between a Dark Septate Endophytic Isolate from Dendrobium sp. and Roots of D. nobile Seedlings

Xiao-Qiang Hou1 and Shun-Xing1*   

  1. 1Institute of Medicinal Plant Development, the Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China
  • Received:2007-09-12 Accepted:2008-06-19 Published:2009-01-31
  • About author:*Author for correspondence Tel: +86 10 6282 9619; Fax: +86 10 6289 5120; E-mail: sxguo2006@yahoo.com.cn
  • Supported by:
    Supported by the National Science Fund of China for Distinguished Young Scholars (30325047).

Abstract:

Interactions between an isolate of dark septate endophytes (DSE) and roots of Dendrobium nobile Lindl. seedlings are reported in this paper. The isolate was obtained from orchid mycorrhizas on Dendrobium sp. in subtropical forest. The fungus formed typical orchid mycorrhiza in aseptic co culture with D. nobile seedlings on modifiedMurashige-Skoog (MMS) medium. Anatomic observations of the infected roots showed that the DSE hyphae invaded the velamen layer, passed through passage cells in exodermis, entered the cortex cells, and then formed fungal pelotons of orchid mycorrhiza. D. nobile seedlings’ plant height, stem diameter, new roots number and biomass were greatly enhanced by inoculating the fungus to seedlings. The fungus was identified as Leptodontidium by sequencing the polymerase chain reaction-amplified rDNA ITS1-5.8S-ITS2 (internal transcribed spacer (ITS)) regions and comparison with similar taxa.

Hou XQ, Guo SX (2009) Interaction between a dark septate endophytic isolate from Dendrobium sp. and roots of D. nobile seedlings. J. Integr. Plant Biol. 51(4), 374–381.

Key words: dark septate endophytes, Dendrobium nobile, fungal inoculation, internal transcribed spacer, molecular identification, orchid mycorrhiza, plant growth.

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