J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (4): 409-413.DOI: 10.1111/j.1744-7909.2008.00785.x

• Molecular Physiology • Previous Articles     Next Articles

High-sensitivity Detection of Fruit Tree Viruses Using Bacterial Magnetic Particles

Ji-Feng Chen1,3,4, Ying Li2, Zheng-Fang Wang2, Ji-Lun Li2, Wei Jiang2 and Shao-Hua Li1,4**   

  1. 1Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
    2Department of Microbiology and Immunology, China Agricultural University, Beijing 100094, China
    3Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China
    4Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan 430074, China
  • Received:2008-05-07 Accepted:2008-08-24 Published:2009-03-30
  • About author:* *Author for correspondence Tel/Fax: +86 10 6283 6026; E-mail: shhli@ibcas.ac.cn Tel/Fax: +86 27 8751 0599; E-mail: shhli@wbgcas.cn

Abstract:

Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay using bacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration (1 × 106 dilution of the original sample concentration) could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude (106) higher than an ELISA-based method of detection PNRSV and GFLV.

Chen JF, Li Y, Wang ZF, Li JL, Jiang W, Li SH (2009) High-sensitivity detection of fruit tree viruses using bacterial magnetic particles. J. Integr. Plant Biol. 51(4), 409–413.

Key words: Bacterial magnetic particle, detection sensitivity, ELISA, fluoroimmunoassay, grapevine fanleaf virus, prunus necrotic ring spot virus.

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