Abstract:

The ¹H nuclear magnetic resonance (¹H NMR) fingerprints of fractionated non-polar extracts (control substance for a plant drug (CSPD) A) from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., of seven specimens from different sources were measured on Fourier Transform (FT)-NMR spectrometer and assigned by comparing them with the 1H NMR spectra of the isolated pure compounds. The ¹H NMR fingerprints showed exclusively characteristic resonance signals of the major special constituents of the plant. Although the differences in the relative intensity of the ¹H NMR signals due to a discrepancy in the ratio of the major constituents among these samples could be confirmed by high performance liquid chromatography analysis, the general features of the ¹H NMR fingerprint established for an authentic sample of the rhizomes of L. chuanxiong exhibited exclusive data from those special compounds and can be used for authenticating L. Chuanxiong species.

Qin HL, Deng AJ, Du GH, Wang P, Zhang JL, Li ZH (2009). Fingerprinting analysis of Rhizoma chuanxiong of commercial types using ¹H nuclear magnetic resonance spectroscopy and high performance liquid chromatography method. J. Integr. Plant Biol. 51(6), 537–544.

Key words: control substance for a plant drug A, ¹H nuclear magnetic resonance fingerprint, high performance liquid chromatography, Ligusticum chuanxiong, phenylpropenoid, phthalides.