J Integr Plant Biol ›› 2009, Vol. 51 ›› Issue (6): 537-544.DOI: 10.1111/j.1744-7909.2009.00831.x

• Metabolism and Biochemistry • Previous Articles     Next Articles

Fingerprinting Analysis of Rhizoma Chuanxiong of Commercial Types using 1H Nuclear Magnetic Resonance Spectroscopy and High Performance Liquid Chromatography Method

Hai-Lin Qin*, An-Jun Deng, Guan-Hua Du, Peng Wang, Jin-Lan Zhang and Zhi-Hong Li   

  1. Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources of Chinese Herbal Medicine, Ministry of Education, Beijing 100050, China
  • Received:2008-11-03 Accepted:2009-03-01 Published:2009-06-08
  • About author: *Author for correspondence Tel: +86 10 8317 2503; Fax: +86 10 6301 7757; E-mail: qinhailin@imm.ac.cn

Abstract:

The 1H nuclear magnetic resonance (1H NMR) fingerprints of fractionated non-polar extracts (control substance for a plant drug (CSPD) A) from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., of seven specimens from different sources were measured on Fourier Transform (FT)-NMR spectrometer and assigned by comparing them with the 1H NMR spectra of the isolated pure compounds. The 1H NMR fingerprints showed exclusively characteristic resonance signals of the major special constituents of the plant. Although the differences in the relative intensity of the 1H NMR signals due to a discrepancy in the ratio of the major constituents among these samples could be confirmed by high performance liquid chromatography analysis, the general features of the 1H NMR fingerprint established for an authentic sample of the rhizomes of L. chuanxiong exhibited exclusive data from those special compounds and can be used for authenticating L. Chuanxiong species.

Qin HL, Deng AJ, Du GH, Wang P, Zhang JL, Li ZH (2009). Fingerprinting analysis of Rhizoma chuanxiong of commercial types using 1H nuclear magnetic resonance spectroscopy and high performance liquid chromatography method. J. Integr. Plant Biol. 51(6), 537–544.

Key words: control substance for a plant drug A, 1H nuclear magnetic resonance fingerprint, high performance liquid chromatography, Ligusticum chuanxiong, phenylpropenoid, phthalides.

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