J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (8): 751-761.DOI: 10.1111/j.1744-7909.2009.00847.x

Special Issue: Sexual Reproductions

• Research Articles • Previous Articles     Next Articles

A Genome-wide Functional Characterization of Arabidopsis Regulatory Calcium Sensors in Pollen Tubes

Liming Zhou1,2, Ying Fu1,2* and Zhenbiao Yang2,3*   

  1. 1 State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China
    2 China Agricultural University (CAU)-University of California, Riverside (UCR) Joint Center for Biological Sciences and Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China
    3 Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
  • Received:2009-03-09 Accepted:2009-05-10 Published:2009-08-17
  • About author:
    * *Authors for correspondence Tel: +1 951 827 7351; Fax: +1 951 827 4437; +86 10 6273 4395; E-mail: yang@ucr.edu and yingfu@cau.edu.cn

Abstract:

Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. Pollen-specific members of calmodulin (CaM), CaM-like (CML), calcium-dependent protein kinase (CDPK) and calcineurin B-like protein (CBL) families were tagged with green fluorescence protein (GFP) and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK24-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK32-GFP caused severe growth depolarization. CBL2-GFP and CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane (PM), and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes.
 

Zhou L, Fu Y, Yang Z (2009). A genome-wide functional characterization of Arabidopsis regulatory calcium sensors in pollen tubes. J. Integr. Plant Biol. 51(8), 751–761.

Key words: Arabidopsis, calcium sensor, calcium signaling, Rho-related GTPase from plants, subcellular localization, tip growth.

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