J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (3): 273-288.DOI: 10.1111/j.1744-7909.2010.00930.x

• Cell and Developmental Biology • Previous Articles     Next Articles

The Arabidopsis BE1 Gene, Encoding a Putative Glycoside Hydrolase Localized in Plastids, Plays Crucial Roles during Embryogenesis and Carbohydrate Metabolism

Xingchun Wang1,2, Li Xue2, Jiaqiang Sun2 and Jianru Zuo2*   

  1. 1College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China
    2State Key Laboratory of Plant Genomics and National Plant Gene Research Center, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China
  • Received:2009-12-03 Accepted:2009-12-07 Published:2010-02-26
  • About author:*Author for correspondence Fax: +86 10 6487 3428; E-mail: jrzuo@genetics.ac.cn

Abstract:

Carbohydrate metabolism is central to plant growth and development. However, little is known about its role in embryogenesis. Here, we report the characterization of multiple alleles of the BRANCHING ENZYME1 (BE1) gene (also known as EMB2729). The weak allele of be1-3, characterized by positional cloning, carries a single-nucleotide substitution in an exon-intron junction and shows various developmental defects during post-germination growth. This mutation causes a reduced level of BE1 mRNA that, likely generated from cryptically spliced pre-mRNA, contains a Glu-to-Lys substitution at codon 366. In four null alleles, BE1 is disrupted by T-DNA insertions, causing embryo developmental arrests at the heart stage. Light microscopy reveals reduced cell divisions and abnormal cell differentiation, thereby leading to defects in setting up the shoot apical meristem, embryonic vascular tissues and cotyledons. Overexpression of BE1 results in a pleiotropic phenotype, indicating that the fine-tuned BE1 level is crucial for plant growth and development. BE1 encodes a putative glycoside hydrolase that is highly conserved in higher plants. A BE1-GFP fusion protein, which is fully functional in complementing be1 mutants, is localized in plastids. The be1-3 phenotype can be partially rescued by glucose, fructose or sucrose, implying the involvement of BE1 in carbohydrate metabolism in plastids.

Wang X, Xue L, Sun J, Zuo J (2010) The Arabidopsis BE1 gene, encoding a putative glycoside hydrolase localized in plastids, plays crucial roles during embryogenesis and carbohydrate metabolism. J. Integr. Plant Biol. 52(3), 273–288.

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