J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (6): 556-567.DOI: 10.1111/j.1744-7909.2010.00946.x

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

Hydrogen Sulfide Alleviates Aluminum Toxicity in Germinating Wheat Seedlings

Hua Zhang1,2*, Zhu-Qin Tan1, Lan-Ying Hu1, Song-Hua Wang3, Jian-Ping Luo1 and Russell L. Jones2   

  1. 1School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
    2Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
    3Life Science College, Anhui Science and Technology University, Bengbu 233100, China
  • Received:2009-11-12 Accepted:2010-02-07 Published:2010-05-01
  • About author: *Author for correspondence Tel: +86 551 2901 506-8635 and +1 510 280-4140; Fax: +86 551 2901 507; E-mail: zhandzhy@yahoo.com.cn

Abstract:

Protective role of hydrogen sulfide (H2S) on seed germination and seedling growth was studied in wheat (Triticum) seeds subjected to aluminum (Al3+) stress. We show that germination and seedling growth of wheat is inhibited by high concentrations of AlCl3. At 30 mmol/L AlCl3 germination is reduced by about 50% and seedling growth is more dramatically inhibited by this treatment. Pre-incubation of wheat seeds in the H2S donor NaHS alleviates AlCl3-induced stress in a dose-dependant manner at an optimal concentration of 0.3 mmol/L. We verified that the role of NaHS in alleviating Al3+ stress could be attributed to H2S/HS by showing that the level of endogenous H2S increased following NaHS treatment. Furthermore, other sodium salts containing sulfur were ineffective in alleviating Al3+ stress. NaHS pretreatment significantly increased the activities of amylases and esterases and sustained much lower levels of MDA and H2O2 in germinating seeds under Al3+ stress. Moreover, NaHS pretreatment increased the activities of guaiacol peroxidase, ascorbate peroxidase, superoxide dismutase and catalase and decreased that of lipoxygenase. NaHS pretreatment also decreased the uptake of Al3+ in AlCl3-treated seed. Taken together these results suggest that H2S could increase antioxidant capability in wheat seeds leading to the alleviation of Al3+ stress.

Zhang H, Tan ZQ, Hu LY, Wang SH, Luo JP, Jones RL (2010) Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings. J. Integr. Plant Biol. 52(6), 556–567.

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