J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (6): 528-535.DOI: 10.1111/j.1744-7909.2010.00949.x

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology • Previous Articles     Next Articles

Genetic Analysis of ele Mutants and Comparative Mapping of ele1 Locus in the Control of Organ Internal Asymmetry in Garden Pea

Xin Li1, Li-Li Zhuang1, Mike Ambrose M. Phil.2,Catherine Rameau 3, Xiao-He Hu4, Jun Yang4 and Da Luo1,4*   

  1. 1School of Life Science and Biotechnology, Shanghai JiaoTong University, Shanghai 200030, China
    2Department of Crop Genetics, John Innes Centre, Colney, Norwich NR4 7UH, UK
    3Station de Génétique et d'Amélioration des Plantes, Institut J.P. Bourgin, UR 254 INRA, Versailles, France
    4National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
  • Received:2009-12-28 Accepted:2010-02-09 Published:2010-05-17
  • About author:*Author for correspondence Tel: +86 21 5492 4108; Fax: +86 21 5492 4108; E-mail: dluo@sibs.ac.cn

Abstract:

Previous study has shown that during zygomorphic development in garden pea (Pisum sativum L.), the organ internal (IN) asymmetry of lateral and ventral petals was regulated by a genetic locus, SYMMETRIC PETAL 1 (SYP1), while the dorsoventral (DV) asymmetry was determined by two CYC-like TCP genes or the PsCYC genes, KEELED WINGS (K) and LOBED STANDARD 1 (LST1). In this study, two novel loci, ELEPHANT EAR-LIKE LEAF 1 (ELE1) and ELE2 were characterized. These mutants exhibit a similar defect of IN asymmetry as syp1 in lateral and ventral petals, but also display pleiotropic effects of enlarged organ size. Genetic analysis showed that ELE1 and ELE2 were involved in same genetic pathway and the enlarged size of petals but not compound leaves in ele2 was suppressed by introducing k and lst1, indicating that the enlargement of dorsal petal in ele2 requires the activities of K and LST1. An experimental framework of comparative genomic mapping approach was set up to map and clone LjELE1 locus in Lotus japonicus. Cloning the ELE1 gene will shed light on the underlying molecular mechanism during zygomorphic development and further provide the molecular basis for genetic improvement on legume crops.

Li X, Zhuang LL, Ambrose M, Rameau C, Hu XH, Yang J, Luo D (2010) Genetic analysis of ele mutants and comparative mapping of ele1 locus in the control of organ internal asymmetry in garden pea. J. Integr. Plant Biol. 52(6), 528–535.

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