J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (6): 568-577.DOI: 10.1111/j.1744-7909.2010.00957.x

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

Antisense-mediated Depletion of Tomato Endoplasmic Reticulum Omega-3 Fatty Acid Desaturase Enhances Thermal Tolerance

Hua-Sen Wang1,2, Chao Yu1,2, Xian-Feng Tang1, Li-Yan Wang1, Xun-Chun Dong1 and Qing-Wei Meng1*   

  1. 1College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China
    2Zhejiang Forestry University, Hangzhou 310029, China
  • Received:2009-11-28 Accepted:2010-03-23 Published:2010-05-01
  • About author:*Author for correspondence Tel: +86 538 8249 606; Fax: +86 538 8226 399; E-mail: qwmeng@sdau.edu.cn

Abstract:

An endoplasmic reticulum-localized tomato ω-3 fatty acid desaturase gene (LeFAD3) was isolated. The antisense tomato plants were obtained under the control of the cauliflower mosaic virus 35S promoter (35S-CaMV). Northern blot analysis confirmed that the expression of LeFAD3 was inhibited in the tomato genome. Levels of 18:3 decreased and correspondingly levels of 18:2 increased in total lipids of leaves and roots. After heat stress, the fresh weight of the aerial parts of antisense transgenic plants was higher than that of the wild type (WT) plants. The membrane system ultrastructure of chloroplasts in leaf cells and all of the subcellular organelles in the root tips of transgenic plants remained more intact than those of WT. Relative electric conductivity increased less in transgenic plants than in WT. Under heat stress, the maximal photochemical efficiency of photosystem II (Fv/Fm) and the O2 evolution rate decreased more in WT than in transgenic plants. These results suggested that the depletion of LeFAD3 increased the saturation of fatty acids and alleviated high temperature stress.

Wang HS, Yu C, Tang XF, Wang LY, Dong XC, Meng QW (2010) Antisense-mediated depletion of tomato endoplasmic reticulum omega-3 fatty acid desaturase enhances thermal tolerance. J. Integr. Plant Biol. 52(6), 568–577.

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