J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (9): 782-792.DOI: 10.1111/j.1744-7909.2010.00968.x

• Metabolism and Biochemistry • Previous Articles     Next Articles

Fingerprinting Analysis of Saposhnikovia divaricata using 1H Nuclear Magnetic Resonance Spectroscopy and High Performance Liquid Chromatography

Yue-Yang Xin, An-Jun Deng, Guan-Hua Du, Jin-Lan Zhang and Hai-Lin Qin*   

  1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
  • Received:2010-03-07 Accepted:2010-05-05 Published:2010-09-01
  • About author:*Author for correspondence Tel: +86-010-83172503 Fax: +86-010-63017757 E-mail:qinhailin@imm.ac.cn

Abstract:

The 1H nuclear magnetic resonance (1H NMR) fingerprints of fractionated non-polar and polar extracts (control substance for plant drug [CSPD] A and B) from the roots of 12 specimens of Saposhnikovia divaricata (Turcz.) Schischk were achieved with Fourier Transform (FT)-NMR spectrometer and assigned by comparison to each other and to the 1H NMR spectra of the isolated individual compounds. These fingerprints were found to be uniform in terms of the specificity for the implication of all 12 specimens being systematically of the same origin. The uniformity was further affirmed by high performance liquid chromatography (HPLC), which also revealed exactly identical specificity for the identified S. divaricata species with the 1H NMR appearances of corresponding CSPD on the part of the composition of characteristic constituents when comparing to corresponding individual compounds. This investigation unambiguously shows that the specific signals from the chemotaxonomically significant compounds of chromones and coumarins in S. divaricata are exhibited distinctively in the composite features of both 1H NMR fingerprints and HPLC profiles. The 1H NMR and HPLC profiles established can successfully be used as reference for the authentication of the origin of S. divaricata species as well as for chemotaxonomic studies.  

Xin YY, Deng AJ, Du GH, Zhang JL, Qin HL (2010) Fingerprinting analysis of Saposhnikovia divaricata using 1H nuclear magnetic resonance spectroscopy and high performance liquid chromatography. J. Integr. Plant Biol. 52(9), 782–792.

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