J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (9): 774-781.DOI: 10.1111/j.1744-7909.2010.00978.x

• Cell and Developmental Biology •     Next Articles

The Cysteine Pairs in CLV2 are Not Necessary for Sensing the CLV3 Peptide in Shoot and Root Meristems

Xiufen Song, Peng Guo, Chen Li, Chun-Ming Liu*   

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2010-05-20 Accepted:2010-06-11 Published:2010-06-21
  • About author:*Author for correspondence Tel:+86 10 6283 6663 Fax:+86 10 8259 9701 Email:cmliu@ibcas.ac.cn


Receptor-like proteins (RLPs) are involved in both plant defense and developmental processes. Previous genetic and biochemical studies show that the leucine-rich repeat (LRR) receptor-like protein CLAVATA2 (CLV2) functions together with CLAVATA1 (CLV1) and CORYNE (CRN) in Arabidopsis to limit the stem cell number in shoot apical meristem, while in root it acts with CRN to trigger a premature differentiation of the stem cells after sensing the exogenously applied peptides of CLV3p, CLE19p or CLE40p. It has been proposed that disulfide bonds might be formed through two cysteine pairs in the extracellular LRR domains of CLV1 and CLV2 to stabilize the receptor complex. Here we tested the hypothesis by replacing these cysteines with alanines and showed that depletions of one or both of the cysteine pairs do not hamper the function of CLV2 in SAM maintenance. In vitro peptide assay also showed that removal of the cysteine pairs did not affect the perception of CLV3 peptides in roots. These observations allow us to conclude that the formation of disulfide bonds is not needed for the function of CLV2.

Song X, Guo P, Li C, Liu CM (2010) The cysteine pairs in CLV2 are not necessary for sensing the CLV3 peptide in shoot and root meristems. J. Integr. Plant Biol. 52(9), 774–781.

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