J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (10): 891-903.DOI: 10.1111/j.1744-7909.2010.00990.x

• Research Articles • Previous Articles     Next Articles

Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

Yuchul Jung1,2, Jungan Park1,2, Yunjung Choi1, Jin-Gweon Yang1, Donggiun Kim1, Beom-Gi Kim3, Kyunghee Roh3, Dong-Hee Lee4, Chung-Kyoon Auh5,* and Sukchan Lee1,*   

  1. 1Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, South Korea
    2Institute of Life Science and Technology, Sungkyunkwan University, Suwon 440-746, South Korea
    3National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, South Korea
    4Department of Life Science, Ewha Womans University, Seoul, 120-750 South Korea
    5Department of Biological Sciences, Mokpo National University, Muan 534-729, South Korea
  • Received:2010-05-17 Accepted:2010-07-28 Published:2010-10-01
  • About author:**Corresponding authors S. Lee, Tel: +82 31 290 7866; Fax: +82 31 290 7892; E-mail: sukchan@skku.ac.kr C.K. Auh, Tel: +82 61 450 2342; Fax: +82 61 454 0267; E-mail:ckauh@mokpo.ac.kr

Abstract:

Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCl. The expression of Δ1-pyrroline-5-carboxylate synthetase was not affected by NaCl stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCl. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCl. Mannitol induced L-proline accumulation more rapidly than NaCl or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline.

Jung Y, Park J, Choi Y, Yang JG, Kim D, Kim BG, Roh K, Lee DH, Auh CK, Lee S (2010) Expression analysis of proline metabolism-related genes from halophyte Arabis stelleri under osmotic stress conditions. J. Integr. Plant Biol. 52(10), 891–903.

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