J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (12): 1075-1085.DOI: 10.1111/j.1744-7909.2010.00992.x

Special Issue: Rice Genomics and Agriculture

• Plant-environmental Interactions • Previous Articles     Next Articles

Proteomic Study Identifies Proteins Involved in Brassinosteroid Regulation of Rice Growth

Fengru Wang1,2,6, Ming-Yi Bai3, Zhiping Deng3, Juan A. Oses-Prieto4, Alma L. Burlingame4, Tiegang Lu5, Kang Chong1* and Zhi-Yong Wang3*   

  1. 1Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
    2Agriculture University of Hebei, Baoding 071001, China
    3Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA
    4Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, University of California, San Francisco CA 94143, USA
    5Biotechnology Research Institute, the Chinese Academy of Agriculture Sciences, Beijing 100081, China
    6Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-05-19 Accepted:2010-08-08 Published:2010-08-26
  • About author:
    **Corresponding authors Kang Chong: Tel: +86 10 6283 6517; Fax: +86 10 8259 4821; E-mail:chongk@ibcas.ac.cn; Zhi-Yong Wang: Tel: +1 650 325 1521 ext 205; Fax: +1 650 325 6857;zywang24@stanford.edu

Abstract:

Brassinosteroids (BRs) are essential hormones for growth and development of plant. In rice, BRs regulate multiple developmental processes and affect many important traits such as height, leaf angle, fertility and seed filling. We identified brassinosteroid-regulated proteins in rice using proteomic approaches and performed functional analysis of some BR-regulated proteins by overexpression experiments. Using two-dimensional difference gel electrophoresis (2-D DIGE) followed by protein identification by mass spectrometry, we compared proteomic differences in the shoots and roots of the BR-insensitive mutant d61-4 and BR-deficient mutant brd1-3. We identified a large number of proteins differentially expressed in the mutants compared with wild type control. These include a glycine-rich RNA-binding protein (OsGRP1) and a DREPP2 protein, which showed reduced levels in the BR mutants. Overexpression of these two proteins partially suppressed the dwarf phenotype of the Arabidopsis BR-insensitive mutant bri1-5. In contrast to the reduced protein level, the RNA level of OsGRP1 was not significantly affected in the BR mutants or by BR treatment, suggesting BR regulation of OsGRP1 at the posttranslational level. This study identifies many BR-regulated proteins and demonstrates that OsGRP1 functions downstream in the BR signal transduction pathway to promote cell expansion.

Wang F, Bai MY, Deng Z, Oses-Prieto JA, Burlingame AL, Lu T, Chong K, Wang ZY (2010) Proteomic study identifies proteins involved in brassinosteroid regulation of rice growth. J. Integr. Plant Biol. 52(12), 1075–1085.

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