J Integr Plant Biol ›› 2011, Vol. 53 ›› Issue (1): 74-88.DOI: 10.1111/j.1744-7909.2010.01010.x

• Plant Reproduction Biology • Previous Articles     Next Articles

WBC27, an Adenosine Tri-phosphate-binding Cassette Protein, Controls Pollen Wall Formation and Patterning in Arabidopsis

Xiao-Ying Dou1, Ke-Zhen Yang1, Yi Zhang2, Wei Wang1, Xiao-Lei Liu1, Li-Qun Chen1, Xue-Qin Zhang1 and De Ye1*   

  1. 1State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
    2Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Southwest University, Chongqing 400716, China
  • Received:2010-09-07 Accepted:2010-11-09 Published:2010-11-18
  • About author:*Corresponding author
    Tel(Fax): +86 10 6273 4839; E-mail: yede@cau.edu.cn

Abstract:

In flowering plants, the exine components are derived from tapetum. Despite its importance to sexual plant reproduction, little is known about the translocation of exine materials from tapetum to developing microspores. Here we report functional characterization of the arabidopsis WBC27 gene. WBC27 encodes an adenosine tri-phosphate binding cassette (ABC) transporter and is expressed preferentially in tapetum. Mutation of WBC27 disrupted the exine formation. The wbc27 mutant microspores began to degenerate once released from tetrads and most of the microspores collapsed at the uninucleate stage. Only a small number of wbc27-1 microspores could develop into tricellular pollen grains. These survival pollen grains lacked exine and germinated in the anther before anthesis. All of these results suggest that the ABC transporter, WBC27 plays important roles in the formation of arabidopsis exine, possibly by translocation of lipidic precursors of sporopollenin from tapetum to developing microspores.

Dou XY, Yang KZ, Zhang Y, Wang W, Liu XL, Chen LQ, Zhang XQ, Ye D (2011) WBC27, an adenosine tri-phosphate-binding cassette protein, controls pollen wall formation and patterning in Arabidopsis. J. Integr. Plant Biol. 53(1), 74–88.

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