J Integr Plant Biol. ›› 2011, Vol. 53 ›› Issue (4): 312-323.DOI: 10.1111/j.1744-7909.2011.01031.x

Special Issue: Rice Genomics and Agriculture

• Molecular Physiology • Previous Articles     Next Articles

Genetic Analysis and Fine Mapping of a Novel Semidominant Dwarfing Gene LB4D in Rice

Fei Liang1,2†, Xiaoyun Xin1,2†, Zejun Hu1,2, Jiandi Xu3, Gang Wei1, Xiaoyin Qian1, Jinshui Yang1, Haohua He2* and Xiaojin Luo1*   

  1. 1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China
    2Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China
    3Institute of Rice Research, Shandong Academy of Agricultural Sciences, Jining 272100, China
  • Received:2010-09-01 Accepted:2011-01-25 Published:2011-02-07
  • About author:**Corresponding authors Tel: +86 791 382 8198; Fax: +86 791 382 8198; E-mail: hhhua64@163.com Tel: +86 21 6564 3715; Fax: +86 216564 3715; E-mail: luoxj@fudan.edu.cn
    These authors contributed equally to this work.


A dwarf mutant, designated LB4D, was obtained among the progeny of backcrosses to a wild rice introgression line. Genetic analysis of LB4D indicated that the dwarf phenotype was controlled by a single semidominant dwarfing gene, which was named LB4D. The mutants were categorized as dn-type dwarf mutants according to the pattern of internode reduction. In addition, gibberellin (GA) response tests showed that LB4D plants were neither deficient nor insensitive to GA. This study found that tiller formation by LB4D plants was decreased by 40% compared with the wild type, in contrast to other dominant dwarf mutants that have been identified, indicating that a different dwarfing mechanism might be involved in the LB4D dominant mutant. The reduction of plant height in F1 plants ranged from 27.9% to 38.1% in different genetic backgrounds, showing that LB4D exerted a stronger dominant dwarfing effect. Using large F2 and F3 populations derived from a cross between heterozygous LB4D and the japonica cultivar Nipponbare, the LB4D gene was localized to a 46 kb region between the markers Indel 4 and Indel G on the short arm of chromosome 11, and four predicted genes were identified as candidates in the target region.

Liang F, Xin X, Hu Z, Xu J,Wei G, Qian X, Yang J, He H, Luo X (2011) Genetic analysis and fine mapping of a novel semidominant dwarfing gene LB4D in rice. J. Integr. Plant Biol. 53(4), 312–323.

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