J Integr Plant Biol. ›› 2012, Vol. 54 ›› Issue (6): 400-411.DOI: 10.1111/j.1744-7909.2012.01126.x

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

Characterization of Two Putative Protein Phosphatase Genes and Their Involvement in Phosphorus Efficiency in Phaseolus vulgaris

Cui-Yue Liang1,2, Zhi-Jian Chen1, Zhu-Fang Yao1, Jiang Tian1* and Hong Liao1   

  1. 1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Root Biology Center, South China Agricultural University, Guangzhou 510642, China
    2Robert Holley Center for Agriculture and Health, United States Department of Agriculture, Agricultural Research Service, Cornell University, Ithaca, New York 14853, USA
  • Received:2011-11-14 Accepted:2012-04-26 Published:2012-05-10
  • About author:*Corresponding author Tel: +86 20 8528 0156; Fax: +86 20 8528 1829; E-mail: jtian@scau.edu.cn

Abstract:

Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses. In this study, two putative protein phosphatases, PvPS2:1 and PvPS2:2 were identified and characterized in bean (Phaseolus vulgaris). The two PvPS2 members were found to be localized to the plasma membrane and the nucleus by transient expression of PvPS2:GFP in onion epidermal cells. Transcripts of the two PvPS2 genes were significantly increased by phosphate (Pi) starvation in the two bean genotypes, G19833 (a P-efficient genotype) and DOR364 (a P-inefficient genotype). However, G19833 exhibited higher PvPS2:1 expression levels than DOR364 in both leaves and roots during Pi starvation. Increased transcription of PvPS2:1 in response to Pi starvation was further verified through histochemical analysis of PvPS2:1 promoter fusion ß-glucuronidase (GUS) in transgenic Arabidopsis plants. Analysis of PvPS2:1 overexpression lines in bean hairy roots and Arabidopsis showed that PvS2:1 was involved in root growth and P accumulation. Furthermore, expression levels of two Pi starvation responsive genes were upregulated and the APase activities were enhanced in the overexpressing PvPS2:1 Arabidopsis lines. Taken together, our results strongly suggested that PvPS2:1 positively regulated plant responses to Pi starvation, and could be further targeted as a candidate gene to improve crop P efficiency.

Liang CY, Chen ZJ, Yao ZF, Tian J, Liao H (2012) Characterization of two putative protein phosphatase genes and their involvement in phosphorus efficiency in Phaseolus vulgaris. J. Integr. Plant Biol. 54(6), 400–411.

Key words: Phosphorus efficiency, HAD protein, acid phosphatase, bean

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