J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (3): 262-276.DOI: 10.1111/jipb.12013

• Metabolism and Biochemistry • Previous Articles     Next Articles

Physiological and Molecular Features of Puccinellia tenuiflora Tolerating Salt and Alkaline-Salt Stress

Xia Zhang1,2†, Liqin Wei1†, Zizhang Wang1 and Tai Wang1*   

  1. 1Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, Beijing 100093, China
    2Graduate School of Chinese Academy of Sciences, 19A Yuquanlu Shijingshanqu, Beijing 100049, China
  • Received:2012-08-06 Accepted:2012-11-06 Published:2013-02-04
  • About author:These authors contributed equally to this work.
    *Corresponding author Tel: +86 10 6283 6210; Fax: +86 10 6259 4170; E-mail: twang@ibcas.ac.cn

Abstract:

Saline-alkali soil seriously threatens agriculture productivity; therefore, understanding the mechanism of plant tolerance to alkaline-salt stress has become a major challenge. Halophytic Puccinellia tenuiflora can tolerate salt and alkaline-salt stress, and is thus an ideal plant for studying this tolerance mechanism. In this study, we examined the salt and alkaline-salt stress tolerance of P. tenuiflora, and analyzed gene expression profiles under these stresses. Physiological experiments revealed that P. tenuiflora can grow normally with maximum stress under 600 mmol/L NaCl and 150 mmol/L Na2CO3 (pH 11.0) for 6 d. We identified 4,982 unigenes closely homologous to rice and barley. Furthermore, 1,105 genes showed differentially expressed profiles under salt and alkaline-salt treatments. Differentially expressed genes were overrepresented in functions of photosynthesis, oxidation reduction, signal transduction, and transcription regulation. Almost all genes downregulated under salt and alkaline-salt stress were related to cell structure, photosynthesis, and protein synthesis. Comparing with salt stress, alkaline-salt stress triggered more differentially expressed genes and significantly upregulated genes related to H+ transport and citric acid synthesis. These data indicate common and diverse features of salt and alkaline-salt stress tolerance, and give novel insights into the molecular and physiological mechanisms of plant salt and alkaline-salt tolerance.

Zhang X, Wei L, Wang Z, Wang T (2013) Physiological and molecular features of Puccinellia tenuiflora tolerating salt and alkaline-salt stress. J. Integr. Plant Biol. 55(3), 262–276.

Key words: Puccinellia tenuiflora, halophyte, salt and alkaline-salt tolerance, microarray assay, gene expression profiles

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