J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (1): 75-82.DOI: 10.1111/jipb.12016

• Research Articles • Previous Articles     Next Articles

Small Ubiquitin-Like Modifier Conjugating Enzyme with Active Site Mutation Acts as Dominant Negative Inhibitor of SUMO Conjugation in Arabidopsis

Konstantin Tomanov1, Christian Hardtke2, Ruchika Budhiraja3, Rebecca Hermkes3, George Coupland3 and Andreas Bachmair1,3*   

  1. 1Department of Biochemistry and Cell Biology, Max F. Perutz Laboratories, Center for Molecular Biology, University of Vienna, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
    2Department of Plant Molecular Biology, University of Lausanne, CH-1015 Lausanne, Switzerland
    3Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany
  • Received:2012-09-08 Accepted:2012-11-19 Published:2013-01-01
  • About author:*Corresponding author Tel: +43 1 4277 74811; Fax: +43 1 4277 974; E-mail: andreas.bachmair@univie.ac.at

Abstract:

Small ubiquitin-like modifier (SUMO) conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.

Key words: SUMO conjugation, SUMO conjugating enzyme, dominant-negative active site mutation, Agroinfection

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