J Integr Plant Biol. ›› 2014, Vol. 56 ›› Issue (7): 695-708.DOI: 10.1111/jipb.12179

• Plant-environmental Interactions • Previous Articles     Next Articles

Quantitative genetic analysis of chlorophyll a fluorescence parameters in maize in the field environments

Domagoj Šimić1†, Hrvoje Lepeduš2†*, Vlatka Jurković1, Jasenka Antunović3 and Vera Cesar3   

  1. 1Agricultural Institute Osijek, HR-31103, Osijek, Croatia
    2Faculty of Humanities and Social Sciences, HR-31000 Osijek, Croatia
    3Department of Biology, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia
  • Received:2014-01-31 Accepted:2014-02-11 Published:2014-02-13
  • About author:These authors contributed equally to this work.
    *Correspondence: E-mail: hlepedus@yahoo.com


Chlorophyll fluorescence transient from initial to maximum fluorescence (“P” step) throughout two intermediate steps (“J” and “I”) (JIP-test) is considered a reliable early quantitative indicator of stress in plants. The JIP-test is particularly useful for crop plants when applied in variable field environments. The aim of the present study was to conduct a quantitative trait loci (QTL) analysis for nine JIP-test parameters in maize during flowering in four field environments differing in weather conditions. QTL analysis and identification of putative candidate genes might help to explain the genetic relationship between photosynthesis and different field scenarios in maize plants. The JIP-test parameters were analyzed in the intermated B73 × Mo17 (IBM) maize population of 205 recombinant inbred lines. A set of 2,178 molecular markers across the whole maize genome was used for QTL analysis revealing 10 significant QTLs for seven JIP-test parameters, of which five were co-localized when combined over the four environments indicating polygenic inheritance and pleiotropy. Our results demonstrate that QTL analysis of chlorophyll fluorescence parameters was capable of detecting one pleiotropic locus on chromosome 7, coinciding with the gene gst23 that may be associated with efficient photosynthesis under different field scenarios.


Šimic D, Lepeduš H, Jurkovic V, Antunovic J, Cesar V (2014) Quantitative genetic analysis of chlorophyll a fluorescence parameters in maize in the field environments. J Integr Plant Biol 56: 695–708. doi: 10.1111/jipb.12179

Key words: Chlorophyll a fluorescence parameters, Field conditions, IBM recombinant inbred lines, JIP-test, Maize (Zea mays L.), Quantitative trait loci (QTL)

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