J Integr Plant Biol. ›› 2014, Vol. 56 ›› Issue (9): 864-875.DOI: 10.1111/jipb.12274

• Research Articles • Previous Articles     Next Articles

Metabolomics-assisted refinement of the pathways of steroidal glycoalkaloid biosynthesis in the tomato clade

Kevin Schwahn1, Leonardo Perez de Souza2, Alisdair R. Fernie2 and Takayuki Tohge2*   

  1. 1University of Potsdam, AG Bioinformatics, Institute for Biochemistry and Biology, Potsdam-Golm, Germany
    2Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
  • Received:2014-06-14 Accepted:2014-08-07 Published:2014-09-01
  • About author:*Correspondence: E-mail: Tohge@mpimp-golm.mpg.de

Abstract:

Steroidal glycoalkaloids (SGAs) are nitrogen-containing secondary metabolites of the Solanum species, which are known to have large chemical and bioactive diversity in nature. While recent effort and development on LC/MS techniques for SGA profiling have elucidated the main pathways of SGA metabolism in tomato, the problem of peak annotation still remains due to the vast diversity of chemical structure and similar on overlapping of chemical formula. Here we provide a case study of peak classification and annotation approach by integration of species and tissue specificities of SGA accumulation for provision of comprehensive pathways of SGA biosynthesis. In order to elucidate natural diversity of SGA biosynthesis, a total of 169 putative SGAs found in eight tomato accessions (Solanum lycopersicum, S. pimpinellifolium, S. cheesmaniae, S. chmielewskii, S. neorickii, S. peruvianum, S. habrochaites, S. pennellii) and four tissue types were used for correlation analysis. The results obtained in this study contribute annotation and classification of SGAs as well as detecting putative novel biosynthetic branch points. As such this represents a novel strategy for peak annotation for plant secondary metabolites.

 

Schwahn K, de Souza LP, Fernie AR, Tohge T (2014) Metabolomics‐assisted refinement of the pathways of steroidal glycoalkaloid biosynthesis in the tomato clade. J Integr Plant Biol 56: 864–875. doi: 10.1111/jipb.12274

Key words: Fruit ripening, glycoalkaloids, secondary metabolite, Solanum lycopersicum, tomato accessions

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